Streptavidin Protein (Atto 425)

Produktnummer ABIN1045077

Hersteller Produkt- Nr.: s000-51

Dieses Native Streptavidin-Protein wird in Streptomyces avidinii exprimiert.

Kurzübersicht für Streptavidin Protein (Atto 425) (ABIN1045077)

Target: Streptavidin

Protein-Typ: Native

Spezies: Streptomyces avidinii

Quelle: Streptomyces avidinii

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA, Dot Blot (DB), Immunofluorescence (IF)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Streptavidin Protein ist gelabelt mit Atto 425.

Hersteller Produkt- Nr.: s000-51

Hersteller: Rockland

Verwendungszweck: Streptavidin ATTO 425 Conjugated

Aufreinigung: STREPTAVIDIN ATTO 425 was prepared from chromatographically purified Streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Streptavidin. No reaction was observed against anti-Avidin.

Anwendungsinformationen

Applikationshinweise:

Application Note: Streptavidin ATTO 425 has been tested by dot blot, western blot, and immunofluorescence. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Western Blot Dilution: >1:10,000

FLISA Dilution: >1:20,000

IF Microscopy Dilution: >1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent) (in 500 μL)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable Expiration date is one (1) year from date of receipt.

Haltbarkeit: 12 months

Antigendetails

Target: Streptavidin

Hintergrund:

Synonyms: SA, S avidin, streptococcus avidin, streptavidin AT425, ATTO 425, ATTO-TEC 425, STREPTAVIDIN ATTO 425 Conjugated

Background: STREPTAVIDIN ATTO 425 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, uti